Journal: Cancers
Article Title: Radiosynthesis and Preclinical Evaluation of 18 F-Labeled Estradiol Derivatives with Different Lipophilicity for PET Imaging of Breast Cancer
doi: 10.3390/cancers16152639
Figure Lengend Snippet: HPLC analysis of the stability of 18 F-TA-Glyco-EE. ( a ) The stability of 18 F-TA-Glyco-EE in human serum was determined after various incubation periods, indicating time-dependent cleavage of the acetyl groups. ( b ) The incubation of MCF-7 cells with 18 F-TA-Glyco-EE for 90 min at 37 °C confirmed that intact 18 F-TA-Glyco-EE was intracellularly detected, without significant deacetylation in the absence of serum.
Article Snippet: HPLC was performed on an Agilent 1100 system (Agilent Technologies Inc., Santa Clara, CA, USA) with a quaternary pump and variable wavelength detector and radio-HPLC detector HERM LB 500 (Berthold Technologies GmbH & Co. KG, Bad Wildbad, Germany).
Techniques: Incubation